Skip to main content
. Author manuscript; available in PMC: 2021 May 18.
Published in final edited form as: Analyst. 2020 May 18;145(10):3517–3527. doi: 10.1039/d0an00383b

Table 3.

Images of dried spots employing different type of blocking buffer with 5% DEX containing IgG FITC-antibodies. A scale bar was 1000 μm. Performing of ATPS ELISA was carried out with information, LOD, CV (%) and S/N±SD (n=3) obtaining by using each blocking buffer as listed below.

Parameter 3× StabilCoat 1× StabilCoat 5%BSA 5%Goat
serum		 0.1%Chonblock/
0.05%goat serum
Bright field images graphic file with name nihms-1583342-t0013.jpg graphic file with name nihms-1583342-t0014.jpg graphic file with name nihms-1583342-t0015.jpg graphic file with name nihms-1583342-t0016.jpg graphic file with name nihms-1583342-t0017.jpg
Fluorescence images graphic file with name nihms-1583342-t0018.jpg graphic file with name nihms-1583342-t0019.jpg graphic file with name nihms-1583342-t0020.jpg graphic file with name nihms-1583342-t0021.jpg graphic file with name nihms-1583342-t0022.jpg
Chemilumine-scence value of the background ±SD (AU) 200,000±5,000 300,000±8,000 200,000±100,000 400,000±60,000 2,000,000±200,000
LOD (pg mL−1) ~1 20 100 60 100
CV (%) 2 3 30 10 10
S/N±SD 28.0±0.6 26.0±0.5 30.0±15.0 10.0±3.0 3.7±0.3