Table 4.
Comparison of three ELISA methods (i.e. standard ELISA, two-incubation ATPS ELISA and one-incubation ATPS ELISA). Comparison of LOD, CV (%) and S/N±SD (n=36) in each method for detection of five cytokines.
| Methods | IL-6 | IL-10 | TNF-α | IL-1β | CCL18 |
|---|---|---|---|---|---|
| LOD (pg mL−1) | |||||
| Standard ELISA | 9.4 | 31.2 | 15.6 | 3.9 | 7.8 |
| Two-incubation ATPS ELISA | 28.6 | 83.5 | 23.0 | 60.7 | 33.0 |
| One-incubation ATPS ELISA | 1.8 | 4.9 | 2.4 | 7.6 | 3.7 |
| S/N | |||||
| Standard ELISA | 30.1 | 15.0 | 10.1 | 30.1 | 33.2 |
| Two-incubation ATPS ELISA | 29.7 | 3.2 | 14.3 | 18.6 | 26.0 |
| One-incubation ATPS ELISA | 30.3 | 10.1 | 27.2 | 43.1 | 43.5 |
| %CV | |||||
| Two-incubation ATPS ELISA | 5.5% | 4.7% | 7.5% | 9.8% | 8.9% |
| One-incubation ATPS ELISA | 6.6% | 4.8% | 6.4% | 8.4% | 8.8% |