. Author manuscript; available in PMC: 2021 May 18.

Published in final edited form as: Analyst. 2020 May 18;145(10):3517–3527. doi: 10.1039/d0an00383b

Table 5.

Summary of the investigated range and selected condition for one-incubation ATPS ELISA.

Variable	Investigated range	Selected condition
1. PEG-DEX concentration	9%-0.81%, 5%-0.81%, 9%-0.45%, 5%-0.45%, 5%-0.27%, 3%-0.45% and 3%-0.27% (%w/w)	5%-0.45% (%w/w)
2. Incubation time	15 minutes, 1 hour and 4 hours	1 hour
3. Types of blocking buffer	3×StabilCoat, 1×StabilCoat, 5%BSA, 0.5%Goat serum and 0.1%Chonblock/0.05%goat serum	3×StabilCoat
4. cAb concentration	2, 4, 6, 8, 10, 15, 20 and 25 μg mL⁻¹	10 μg mL⁻¹
5. dAb concentration	1, 10, 25, 50 and 75 ng mL⁻¹	10 ng mL⁻¹ for IL-6, 25 ng mL⁻¹ for IL-10, TNF-α, IL-1β and CCL18
6. HRP concentration	1×, 3×, 5×, 10× and 15×	5×