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. 2020 May 19;10:8286. doi: 10.1038/s41598-020-64623-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of MACROD1 leads to altered mitochondrial morphology. (a) HeLa cells were seeded on Ibidi 8-well slides and transfected with indicated siRNAs and a plasmid containing mTurquoise2 targeted to mitochondria. Cells were analysed live using confocal microscopy. A representative image of 3 independent experiments is shown. (b) Automated quantification of at least 50 cells per condition using the mitochondrial network analysis (MiNA) macro tool in ImageJ. Scale bars represent 10 µM. Statistical analysis was performed by a Wilcoxon rank-sum test for comparison of MACROD1 siRNA treated cells with siCTRL treated cells (ns = not statistically significant (P > 0.05), ***P < 0.001, ****P < 0.0001).