Table 2.
Antibodies recommended for Western Blot and Immunofluorescence.
| Application | Antigen | Antibody name | Remarks |
|---|---|---|---|
| Western Blot | |||
| MACROD1 | 25E9 | Shows additional bands; not suitable for IF. Gives a stronger signal than the 28C11 antibody. | |
| MACROD2 | 18D12 or HPA049076 | The HPA antibody recognizes many larger bands in addition to a band at ± 28 kD; not suitable for IF. | |
| TARG1 | 3A5 | Shows an additional band in HeLa Flp-In T-REx | |
| Immunofluorescence | |||
| MACROD1 | 28C11 | Slightly weaker staining than 23E9 but highly specific. | |
| MACROD2 | No suitable antibody was found; the polyclonal antibody used before38 is not available. | ||
| TARG1 | PA5-56043 | This antibody shows the most promising localisation in IF but needs validation in WB. | |
Table 2 summarises the recommended antibodies for Western Blot and immunofluorescence analysis of MACROD1, MACROD2 and TARG1 and describes their limitations.