Figure 6.

Viability of hCh in 3D plotted biphasic interwoven algMC-CPC constructs. (A) Fluorescence images of biphasic scaffolds revealed decreasing cell viability during redifferentiation in close proximity (300 µm) to CPC interface (ii), compared to gel-gel interface (i), scale bar = 500 µm. (B) Via live-dead quantification of cLSM images, no statistically significant difference was found comparing differentiating cells at gel-gel interface to cells within a 300 µm distance to CPC strand (gel-CPC). n ≥ 5. *p < 0.05, mean ± SEM. (C) Brightfield image of strand intersections, and live-dead stained cLSM fluorescence images of hCh (green: calcein (viable), red: ethidium homodimer 1 (dead)) at strand interfaces of algMC strand and CPC strand inside one scaffold, day 21 for ctrl and diff cultivation, scale bar = 250 µm. (D) Quantification of live-dead ratios during 3 weeks of cultivation in ctrl and diff condition, n ≥ 11, ***p < 0.001.