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. 2020 May 19;10:8277. doi: 10.1038/s41598-020-65050-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cytoskeletal morphology, distribution and chondrogenic phenotype of hCh in biphasic interwoven algMC-CPC scaffolds. (A) Fluorescence staining of cell nuclei (blue: DAPI) and cytoskeletal F actin filaments (green: phalloidin) at day 28, scale bar = 500 µm. Cells tended to migrate out of the gel and colonize CPC surface (arrows) in ctrl condition (left, day 28) while redifferentiated cells remained inside the algMC matrix phase (right, day 28). (B) Fluorescence staining of cell nuclei (blue: DAPI), the chondrogenic markers ACN (top, red) or COL2 (bottom, turquoise) and cytoskeletal F actin filaments (green: phalloidin) in chondrocyte clusters of interwoven algMC-CPC scaffolds in close distance to CPC after 21 days, scale bar = 100 µm.