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. 2020 May 19;4:12. doi: 10.1038/s41698-020-0117-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Vertical concentration gradients of fluorescent doxorubicin (DOX, red) and Hoechst dye (blue) in U87 xenograft slices at 4 h a or 48 h b, separated by buffer lanes (white triangles). c Vertical fluorescence profiles above source lanes versus distance from the membrane at 4 h or 48 h. Colored lines represent average fluorescence at different lanes for 4 h (4 DOX, five Hoechst), and at spaced locations on 2 lanes for 48 h (4 DOX, three Hoechst). Gray profiles represent Hoechst for doxorubicin (and vice versa), averaged at 4 h and individual profiles at 48 h. d Horizontal profiles at 48 h show lateral diffusion. Locations indicated in b (lines 1–3). e Lateral fluorescence spread from source lanes at 48 h as indicated in d. Average ± SEM, n = 4 for DOX, n = 3 for Hoechst except for four lanes over. One-way ANOVA, Tukey’s multiple comparison test. **p < 0.0001. Scale bars: 500 μm a, b.