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. 2020 May 19;10:8265. doi: 10.1038/s41598-020-65093-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the four strains used in this study. (a) PS533 (wild-type), (b) Coat-less PS4150 (ΔcotE::tet ΔgerE::spc), (c). DPA-less FB122 (ΔspoVF::tet ΔsleB::spc). The brown layer represents the inner and outer coat, the orange layer the outer membrane, the green layer the cortex, the second orange layer the cell membrane and inner membrane. The core contains DPA, if not deleted. Strain FB113 (ΔcwlJ::tet ΔsleB::spc) in the dormant form has similar structure organization as PS533 (a), and when germinated might be structurally comparable to FB122 (c).