Table 3.

Summary of samples’ effective forces constants as determined by fitting MSDs. Effective force constants were determined at low Q and high Q ranges as specified. If not indicated otherwise, the force constants were extracted from the whole temperature range.

Sample preparation	Strains/sporulation medium	Effective force constant k’ (N/m) Low Q (0.5 < Q < 1.67 Å-1)	Effective force constant k’ (N/m) High Q (1.4 < Q < 2.0 Å-1)
D2O pellets	PS 533 2*SG agar	0.40 ± 0.11	0.21 ± 0.05
	PS 533 2*SG agar (germinated)	0.10 ± 0.02	0.44 ± 0.35
	FB113 2*SG agar	0.38 ± 0.10	0.23 ± 0.05
	FB113 2*SG agar (germinated)	0.26 ± 0.10	0.26 ± 0.09
	FB122 2*SG agar	0.24 ± 0.04	0.19 ± 0.05
H2O pellets	PS533 2*SG liquid	[280–303 K] 0.38 ± 0.14	11 ± 80**
		[303–315 K] 0.05 ± 0.01*
	FB122 2*SG liquid	0.31 ± 0.04	0.47 ± 0.23
D2O hydrated powder	PS533 2*SG liquid	0.25 ± 0.02	0.46 ± 0.15
	PS4150 2*SG liquid	0.23 ± 0.02	0.29 ± 0.04
D2O suspensions	PS533 2*SG liquid	[285–307 K] 0.27 ± 0.11	0.37 ± 0.17
		[307–315 K] 0.028 ± 0.010*
	PS533 2*SG agar	0.29 ± 0.13	0.38 ± 0.15
	PS533 SPZ agar	0.20 ± 0.05	0.24 ± 0.2
	PS4150 SPZ agar	[280–307 K] 0.25 ± 0.16	1.6 ± 1.6**
		[307–315 K] 0.010 ± 0.003*
H2O suspensions	PS533 SPZ agar	0.31 ± 0.04	0.53 ± 0.75

*Calculations of k’ during germination has no physical meaning as the sample is evolving throughout this process but it allows to visualize the large increase in motions amplitude occurring during germination.

**Statistics was not sufficient to perform acceptable fits; as the slope is almost zero, the calculated effective force constant becomes meaningless.