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The accumulation of RNA products from in vitro transcription reactions using flgB promoter dsDNA increases with increasing concentrations of the sigma factor RpoD. RNA was separated by a 10% TBE-urea gel and radiolabeled RNA was detected by phosphor screen. The signal from the phosphor screen was quantified by densitometry. Results are representative of two replicate experiments. R2 for linear regression is reported to indicate RpoD-dependent variability in RNA polymerase activity.
