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. 2020 May 19;10:8292. doi: 10.1038/s41598-020-65169-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Responses mediated by rat P2X2 confirm 2-fluoro-ATP (2F-ATP) is a full agonist. (A) Recordings under whole-cell voltage clamp from HEK cells expressing rat P2X2 show the response to ATP increases with concentration. (B) Responses to ATP, ADP, ATP (repeat stimulus), 2F-ATP and ATP (repeat stimulus) presented sequentially each at 10 μM, relative to ATP at 1 mM (mean and SEM, *P < 0.05 ANOVA with Bonferroni post-test, NS non-significant). (C) Current traces in one of the experiments underlying data in (B). Applications of ADP, 2F-ATP and 1 mM ATP each followed three applications of ATP. (D) Response to 2F-ATP in flies expressing rat P2X2 to increasing concentrations (mean and SEM, N = 10, EC₅₀ = 17.2 μM with 95%CI 9.5 to 31.3 μM). Black dashed curve is fitted to ATP data from Fig. 1F for comparison.