Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 19;10:8292. doi: 10.1038/s41598-020-65169-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Responses mediated by T184I rP2X2::GFP in taste neurons. (A) 100 μM ATP elicits responses from mutated rP2X2 while 100 μM ADP does not. Top traces from control flies carrying a UAS transgene but no driver, middle traces from flies expressing T184I rP2X2 in sugar taste neurons under control of the Gr5a promoter, bottom traces from flies with pan-neural expression of T184I rP2X2 under control of the C155 driver. (B) Mean responses (with SEM) to solvent, ADP and ATP in control flies and in flies expressing T184I rP2X2 in sugar taste neurons and in all neurons (ns p ≥ 0.05 **p < 0.01 ***p < 0.001 paired Anova and post-hoc Dunnett’s test). Solvent elicited impulse rates among genotypes differ significantly between Gr5a-rP2X2^T184I and C155-rP2X2^T184I, p = 0.0001 (unpaired Anova and post-hoc Dunnett’s test). (C) Response to ATP in flies expressing rat P2X2 to increasing concentrations of ATP (mean and SEM, N = 14, EC₅₀ = 145 μM with 95%CI 92 to 370 μM). (D) Light-sheet fluorescence micrograph of neurons with pan-neural expression of T184I rP2X2::GFP (in green).