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. 2020 May 19;10:8285. doi: 10.1038/s41598-020-64873-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Knock-down of Mettl4 leads to decreased 6 mA levels. (a) Phylogenetic tree analysis of METTL4 in mammals. (b) 3T3-L1 cells were transfected with Mettl4-targeting siRNAs. With the cells, gene expression of Mettl4 was determined by quantitative RT-PCR, in relative to control cells treated by a sequence-irrelevant siRNA. (c,d) Genomic 6 mA levels were determined by dot blot (c) and HPLC-MS/MS. Full-length blots are presented in Supplementary Fig. S5 (d). Data are analyzed by Student’s t-test and presented as mean ± s.d., n = 3 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.