Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 19;11:2506. doi: 10.1038/s41467-020-16052-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a RT-PCR validation of aberrant splicing of ESRRG in MCF7 cells transduced with Ad-null, Ad-SF3B1^WT, and Ad-SF3B1^R625H, respectively. The higher band corresponds to the cryptic ESRRG transcript and the lower band corresponds to the canonical ESRRG transcript. b, c Sanger sequence of gel-purified fragment showing the aberrant ESRRG transcript in SF3B1^R625H cells b, the canonical ESRRG transcript in SF3B1^WT cells c. The red dotted lines indicate the location of 21 nucleotides. d Structure of minigene splicing reporters spanning the E5 region of ESRRG. e RT-PCR validation of aberrant splicing of ESRRG in MCF7 cells co-transduced minigene reporter with Ad-null, Ad-SF3B1^WT and Ad-SF3B1^R625H, respectively. The higher band corresponds to the cryptic ESRRG transcript and the lower band corresponds to the canonical ESRRG transcript. f, g Sanger sequence of gel-purified fragment showing the aberrant ESRRG transcript in SF3B1^R625H cells f, the canonical ESRRG transcript in SF3B1^WT cells g.