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. 2020 May 19;10:8231. doi: 10.1038/s41598-020-65069-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transplantation methods of fetal liver tissue onto the liver surface. (a) Schematic of the transplantation of a fetal liver tissue coated with a coating agent. After 27 days of first DMN injection, the samples were collected and analyzed. (b) Histological analysis of liver tissue with a coating agent covering the liver surface before transplantation. DPPIV-stained living tissue is colored in red. Scale bar: 2 mm. (c) Measurement of the survival rate of fetal liver tissue transplantation after coating agent utilization. The survival rate of the transplanted group without coating agent was also monitored as a control. Data are mean ± SE. *P < 0.01, log rank test; no coating: n = 20, coating: n = 15. (d,e) Measurement of the engraftment rate and area of fetal liver tissue transplantation after coating agent utilization. The engraftment rate and area in the transplanted group without coating agent was also monitored as a control. Data are mean ± SE. *P < 0.05, vs. wild-type Mann-Whitney U test, n = 11.