Fig. 1. mIMCD3 cells change their cell cycle ratio when grown in 3D culture.

a Cells growing in a contact-free environment (e.g., a hanging drop from the lid of a cell culture vessel) spontaneously form spheroids. Size marker = 500 μm. b 2D grown cells and cells in 3D culture for 24 and 48 h change their cell cycle distribution over time. Cells in G1 phase expand on the expense of cells in S phase and G2/M phase. c Ki67 serves as a bona fide marker for cells actively participating in cell cycle. Cells negative for Ki67 represent G0 phase cells entered the dormant state. Bars represent mean ± standard deviation of three biologically independent FACS experiments.