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. 2020 May 19;3:246. doi: 10.1038/s42003-020-0973-6

Fig. 5. Lipid composition of cells grown in 2D of 3D or in situ.

Fig. 5

a PCA of lipid profiling with PC1 against PC2. Red: whole kidney lysate, green: cells grown in 2D, blue: cells grown in 3D, turquoise: isolated distal tubules from perfused kidneys, and pink: pooled quality control sample. Replicates are highlighted as individual dots, shaded area shows the 95% confidence interval. b Heat map and cluster analysis of lipids and fatty acids. Range-scaled z-scores of annotated features with a q-value < 0.05 according to ANOVA and FDR correction. Differently abundant metabolite clusters after Pearson and Ward reflect the differences between cultured 2D or 3D grown mIMCD3 cells and kidney lysates. Green: 2D grown cells, red: whole kidney lysate, blue: 3D grown mIMCD3 cells, turquoise: isolated nephrons, n for nephrons = 3, n for 2D, 3D, and kidneys = 4. Identified lipids can be clustered into six subgroups (a–f, identity of the clusters is explained in the main text and Supplementary Fig. 3). A complete list of all identified entities is shown in Supplementary Data 5.