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. 2020 May 19;10:8254. doi: 10.1038/s41598-020-65103-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the strategy for tear glucose detection by measuring the reflectance spectrum. (a) Configuration of the integrating sphere consisting of d/8° geometry. The external light source projects perpendicularly to the sample and the detection port were coupled with spectrometer 8-degree varied from the normal. (b) Photograph of NECL captured before reaction (left) and after reaction with 2.4 mM of glucose solution (right), mixed with artificial tear. Scale bar is 1 mm. (c) Collected light was quantitatively analyzed by this spectroscopic system. The tear glucose concentration was predicted by comparing the reflection spectra of control and reacted contact lens.