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. 2020 May 19;11:2487. doi: 10.1038/s41467-020-16051-9

Fig. 2. SH3RF3 promotes CSC properties of breast cancer cells.

Fig. 2

a SH3RF3 overexpression in HMLE, MCF10AT and MDA-MB-231 cells. b Flow cytometry analyses of the CD44+CD24− subpopulations in HMLE and MCF10AT, and the ALDH+ subpopulation in MDA-MB-231. Numbers in the flow cytometry charts indicate the CSC percentages (n = 3 culturing experiments). c Quantitation and representative images of tumorsphere formation in SH3RF3-overexpressing breast cancer cells (n = 3 culturing experiments). Scale bar,100 μm. d In vivo tumor formation of the mice injected with serial dilutions of MDA-MB-231 cells at day 28. e Tumor images and volumes (n = 10 mice) in mice injected with 40 control and SH3RF3-overexpressing MDA-MB-231 cells. Data represent mean ± SD. Statistical significance was determined by two-tailed unpaired t-test (b and c), chi-squared test (d) or Mann–Whitney U-test (e). The experiments in (a), (b) (upper), and (c) were repeated three times independently with similar results, and the data of one representative experiment are shown. Source data are provided as a Source data file.