Fig. 3. SH3RF3 knockdown impairs CSC traits of breast cancer cells.

a SH3RF3 knockdown in HMLER-CD44H and MCF10CA1h cells. b Flow cytometry analyses of the CD44^high subpopulation in HMLER-CD44H and the ALDH⁺ subpopulation in MCF10CA1h (n = 3 cell culture experiments). c Quantitation and representative images of tumorsphere formation of breast cancer cells with SH3RF3 knockdown (n = 3 sphere culture experiments). Scale bar, 100 μm. d In vivo tumor formation of the mice injected with serial dilutions of MCF10CA1h at day 42. e Tumor images and volumes (n = 8 mice) in mice injected with 200 control or SH3RF3 knockdown MCF10CA1h cells. Data represent mean ± SD. Statistical significance was determined by two-tailed unpaired t-test (b and c), chi-squared test (d) or Mann–Whitney U-test (e). The experiments in (a), (b) (left), and (c) were repeated three times independently with similar results, and the data of one representative experiment are shown. Source data are provided as a Source data file.