Skip to main content
. 2020 May 19;11:2487. doi: 10.1038/s41467-020-16051-9

Fig. 5. PTX3 mediates the function of SH3RF3 in BCSC regulation.

Fig. 5

a Correlation of ssGSEA scores of the gene sets regulated after PTX3 and SH3RF3 overexpression in breast cancer cell lines (n = 51 cell lines). b GSEA analyses of CD44 or YAP-related gene sets in PTX3-overexpressing versus control cells. c Expression heatmap of Hedgehog and Hippo-regulated genes after PTX3 and SH3RF3 overexpression. d PTX3 knockdown in MDA-MB-231 with SH3RF3 overexpression. e Flow cytometry analyses of ALDH+ subpopulation in MDA-MB-231 with SH3RF3 overexpression and PTX3 knockdown. f Quantitation (n = 4 FACS experiments) of ALDH + CSC subpopulations in MDA-MB-231 with SH3RF3 overexpression and PTX3 knockdown. g Tumorsphere quantitation in MDA-MB-231 with SH3RF3 overexpression and PTX3 knockdown (n = 3 culturing experiments). h Tumor initation in the mice injected with 40 cells of MDA-MB-231 cells at day 28. Data represent mean ± SD, n = 3. Statistical significance was determined by paired t-test (a) or two-tailed unpaired t-test (f and g). The experiments in (d), (e), and (g) were repeated three times independently with similar results, and the data of one representative experiment are shown. Source data are provided as a Source data file.