Skip to main content
. 2020 May 19;11:2487. doi: 10.1038/s41467-020-16051-9

Fig. 7. SH3RF3 activates JUN by interacting with MKK and JNK.

Fig. 7

a Co-IP analyses of SH3RF3-JNK1/2 interaction in 293T cells. b Co-IP analyses of SH3RF3-MKK4/7 interaction in 293T cells. c Co-IP analyses of SH3RF3-JIP3 interaction in 293T cells. d Sequential Co-IP analyses of SH3RF3-JIP3-MKK7 interaction in 293T cells. e Co-IP analyses of JIP3-MKK7 interaction in MCF10AT (top), and with or without SH3RF3 overexpression (bottom). f Co-IP analyses of SH3RF3-JNK1/2 interaction in MCF10AT with or without JIP3 knockdown. g JUN phosphorylation after SH3RF3 overexpression and JIP3 knockdown in MCF10AT. h Co-IP analyses of the interactions of various SH3RF3 truncations to MKK7, JIP3, and JNK1. The experiments were repeated three times independently with similar results, and the data of one representative experiment are shown. Source data are provided as a Source data file.