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. 2020 May 19;11:2487. doi: 10.1038/s41467-020-16051-9

Fig. 8. The clinical relevance of SH3RF3 in human breast tumors.

Fig. 8

a Patient-derived organoid formation (n = 3 organoid culture replicates) in a human breast tumor, LTMBC-1, after SH3RF3 overexpression. (Right panel) Lines represent median values and whiskers indicate the standard deviation. Haematoxylin & eosin staining of a representative organoid was shown on the right. Scale bar, 100 μm. b Patient-derived organoid formation (n = 3 organoid culture replicates) of two other human breast tumors (LTMBC-2 and 3) after SH3RF3 overexpression. c Patient-derived organoid formation (n = 3 organoid culture replicates) of four human gastric cancers (LTMGC-1, 2, 4, and 13) with SH3RF3 overexpression. Scale bar, 100 μm. d Correlation of SH3RF3 and PTX3 expression to different CSC marker genes in TCGA breast cancer cohort (n = 1097 patients). e Correlation of SH3RF3 expression and the ssGSEA scores of CSC and MaSC-related gene sets in TCGA breast cancer cohort (n = 1097 patients). f Representative IF images of SH3RF3 and CD44 in breast cancer tissues of the Qilu cohort. Scale bar, 100 μm. g CD44 expression levels in breast cancer tissues of the Qilu cohort with different SH3RF3 expression (n = 40 patients). Numbers in parenthesis indicate sample sizes. h Correlation of SH3RF3 and CD44 IF intensities in breast cancer tissues of the Qilu cohort (n = 40 patients). i Survival analysis by SH3RF3 expression in Kaplan–Meier Plotter breast cancer cohort (n = 626 patients). j Schematic model of the role of SH3RF3 in BCSC regulation. Data represent mean ± SD. Statistical significance was determined by two-tailed unpaired t-test (a, b and c), chi-squared test (g), paired t-test (h) or two-sided log-rank test (i). Source data are provided as a Source Data file.