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Seven experimental factors and the corresponding three levels tested using the fractional factorial design.
| Factors | Code | Level 0 | Level 1 | Level 2 |
|---|---|---|---|---|
| Type of iron salt | A | FeCl3 | FePO4 | FePP |
| pH | B | 3 | 5 | 8 |
| Temperature (°C) | C | 23 | 40 | 100 |
| Ionic strength (mM NaCl) | D | 0 | 100 | 1000 |
| Concentration iron (mM) | E | 1 | 5 | 10 |
| Ratio [Fe]:[Cat] | F | 1:1 | 1:2 | 1:3 |
| MSG (mM) | G | 0 | 125 | 250 |
