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. 2020 May 19;10:8257. doi: 10.1038/s41598-020-64945-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum-likelihood phylogenetic tree of Heterorhabditis nematodes based on 18S ribosomal RNA gene sequences. The evolutionary distances were computed using the Hasegawa-Kishino-Yano model. The tree with the highest log likelihood (−3422.19) is shown. Numbers at nodes represent bootstrap values higher than 70% based on 100 replications. The rate variation among sites was modeled with a gamma distribution (5 categories (+G, parameter = 0.4803)). Bar represents 0.01 nucleotide substitutions per sequence position. Accession numbers of the gene sequences used for the reconstructions and those obtained in this study are available in Tables S1 and S2, respectively.