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. 2020 May 19;10:8251. doi: 10.1038/s41598-020-64821-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the OsO₄, chromic acid and Oil Red O frozen-section techniques to detect fat emboli in the lung. Left images correspond to a fresh sperm whale and right images to an advance decomposed sperm whale. Examples of fat emboli are pointed out with arrows. Asterisks in the right-bottom image point to the surpassing vessel’s limit. Bars = 200 µm, except in the left-bottom image where Bar = 100 µm.