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. 2020 May 13;11:636. doi: 10.3389/fphar.2020.00636

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Copyright © 2020 Lopes, Berg and Tambourgi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PMSF and Monensin inhibit the SMase D-induced shedding of surface markers. HaCaT cells were pre-incubated for 5 min with PMSF (1 mM) or Monensin (10 µg/ml) followed by SMase D (25 µg/ml) or buffer for 2 h. Expression of EGFR (A), β2-microglobulin (B), MCP (C), and TNF-RI (D) was analyzed by flow cytometry. Data are presented as mean ± standard error of duplicates, representative of two independent experiments. Statistically analyzed by one-way ANOVA followed by Tukey HSD test using GraphPad Prism 6.1 Software. (*) Significant difference compared to buffer (p < 0.05). (#) Significant difference in relation to SMase D (p < 0.05).