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. 2020 May 13;11:636. doi: 10.3389/fphar.2020.00636

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Copyright © 2020 Lopes, Berg and Tambourgi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of ADAM-10 and 17 and detection of ERK1/2 phosphorylation in human keratinocytes after treatment with SMase D. HaCaT cells were treated for 2 h with SMase D (25 µg/ml) or buffer and analyzed for the expression of ADAM-10 (A) and ADAM-17 (B) by flow cytometry. Alternatively, cells were treated with SMase D or buffer for 30 and 60 min and ERK1/2 phosphorylation was evaluated by using the EIA kit Titerzime Phospho-ERK1/2 (C). Data are expressed as mean ± standard error of duplicates, representative of two independent experiments. Statistically analyzed by Two Way ANOVA followed by Tukey HSD test for the evaluation of ERK1/2 and analyzed by Student's t test for ADAMs expression, using the GraphPad Prism 5.1. (*) Significant difference compared to buffer (p < 0.05).