FIGURE 8.

HSV-1 UL2 does not block the TNF-α–induced nuclear translocation of p65 or p50. HeLa cells were transfected with HA vector or UL2-HA expression plasmid. Twenty-four hours posttransfection, cells were treated with TNF-α (10 ng/mL) or mock-treated for 30 min. Then, cells were stained with anti-HA mAb and anti-p65 pAb (A) or anti-p50 pAb (B). Fluorescein isothiocyanate–conjugated donkey anti–mouse IgG (green) and Cy5-conjugated goat anti–rabbit IgG (red) were used as the secondary Abs. Cell nuclei were stained with DAPI (blue). All of the transfected cells were analyzed by a confocal microscope (Axio-Imager-LSM-800; Zeiss), and the photomicrographs were taken at a magnification of 400×. Each image represented a vast majority of the cells with similar subcellular distribution. Statistical analysis of the subcellular localization of p65 or p50 in the absence or presence of UL2 is shown in Table 2.