TABLE 1.
Primers used for constructing the recombinant HSV-1 UL2 plasmids merged with EYFP.
| Plasmids’ name | Primer name | Forward primer (5′→3′) |
| UL2 (1–334)-EYFP | UL2-HindIII,EcoRI-F | CGAAGCTTCGGAATTCATGAAGCGGGCCTGCAGCCG |
| UL2-HindIII,BamHI-R | GCAAGCTTAGGATCCGTAACCGACCAGTCGATGGGTG | |
| UL2 (1–8)-EYFP | UL2 (1–8)-EcoRI-F | AATTCATGAAGCGGGCCTGCAGCCGAAGCTTG |
| UL2 (1–8)-BamHI-R | GATCCAAGCTTCGGCTGCAGGCCCGCTTCATG | |
| UL2 (1–17)-EYFP | UL2 (1–17)-EcoRI-F | AATTCATGAAGCGGGCCTGCAGCCGAAGCCCCTCACCACGCCGCCGCCCATCATCGTTG |
| UL2 (1–17)-BamHI-R | GATCCAACGATGATGGGCGGCGGCGTGGTGAGGGGCTTCGGCTGCAGGCCCGCTTCATG | |
| UL2 (9–17)-EYFP | UL2 (9–17)-EcoRI-F | AATTCATGCCCTCACCACGCCGCCGCCCATCATCGTTG |
| UL2 (9–17)-BamHI-R | GATCCAACGATGATGGGCGGCGGCGTGGTGAGGGCATG | |
| UL2 (1–31)-EYFP | UL2-HindIII,EcoRI-F | CGAAGCTTCGGAATTCATGAAGCGGGCCTGCAGCCG |
| UL2 (1–31)-BamHI-R | TTGGATCCAATTTTTGCGGCGGCGTCCCGTC | |
| UL2 (61–75)-EYFP | UL2 (61–75)-EcoRI-F | AATTCATGCGCTCGTCAGGGCCGGCGGGCGCTCCTCGCCGCCCTAGAGGCTGTATG |
| UL2 (61–75)-BamHI-R | GATCCATACAGCCTCTAGGGCGGCGAGGAGCGCCCGCCGGCCCTGACGAGCGCATG | |
| UL2 (69–75)-EYFP | UL2 (69–75)-EcoRI-F | AATTCATGCCTCGCCGCCCTAGAGGCTGTTCG |
| UL2 (69–75)-BamHI-R | GATCCGAACAGCCTCTAGGGCGGCGAGGCATG | |
| UL2 (69–224)-EYFP | UL2 (69–224)-EcoRI-F | TTGAATTCATGCCTCGCCGCCCTAGAGGCTG |
| UL2 (69–224)-BamHI-R | AAGGATCCGTGCAACCGTGGCCGCTCATCCG | |
| UL2 (225–334)-EYFP | UL2 (225–334)-EcoRI-F | TTGAATTCATGCTGGAAAAGTGGGCGCGGGAC |
| UL2-HindIII,BamHI-R | GCAAGCTTAGGATCCGTAACCGACCAGTCGATGGGTG | |
| UL2 (225–240)-EYFP | UL2 (225–334)-EcoRI-F | TTGAATTCATGCTGGAAAAGTGGGCGCGGGAC |
| UL2 (225–240)-BamHI-R | CGGGATCCAACAGGGTCGTGTTTAGTAACAG | |
| UL2 (225–277)-EYFP | UL2 (225–334)-EcoRI-F | TTGAATTCATGCTGGAAAAGTGGGCGCGGGAC |
| UL2 (225–277)-BamHI-R | AAGGATCCAAGAGCATAAACACCAGGCCGGG | |
| UL2 (278–334)-EYFP | UL2 (278–334)-EcoRI-F | TTGAATTCATGTGGGGCGCACATGCCCAGAAT |
| UL2-HindIII,BamHI-R | GCAAGCTTAGGATCCGTAACCGACCAGTCGATGGGTG | |
| UL2 (9–17) del -EYFP | UL2 (9–17) del -EcoRI-F | TTGAATTCTATGAAGCGGGCCTGCAGCCGAAGCCCACGTTGGACCCCACCC |
| UL2-HindIII,BamHI-R | GCAAGCTTAGGATCCGTAACCGACCAGTCGATGGGTG | |
| UL2 (9–17) (69–75) del -EYFP | UL2 (9–17) del -EcoRI-F | TTGAATTCTATGAAGCGGGCCTGCAGCCGAAGCCCACGTTGGACCCCACCC |
| UL2 (69–75) del-R | GAAAACGTCACACCAGCGGGAGCGCCCGCCGGCCCTGACGAG | |
| UL2 (69–75) del-F | CCCGCTGGTGTGACGTTTTC | |
| UL2-HindIII,BamHI-R | GCAAGCTTAGGATCCGTAACCGACCAGTCGATGGGTG |