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. 2020 May 19;10:8259. doi: 10.1038/s41598-020-65269-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Multiple cell subsets are present in the inflamed skin. (A,B) Gating strategy for identification of myeloid (A) and lymphoid (B) subsets in the skin. (C–E) Total number of leukocytes (C), lymphoid (D) and other cells types (E) in the ear WT and K23 mice 20 weeks after TAM treatment. Neutrophils (neut), macrophages (macr), inflammatory monocytes (infl mono), natural killer cells (NK), eosinophils (eos). n = 5–7 mice/group. Data are shown as mean± SEM, *p < 0.05, **p < 0.01 by nonparametric Mann-Whitney test.