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. 2020 May 19;10:8239. doi: 10.1038/s41598-020-65067-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fibre type composition in developmental, mature, and aged soleus and plantaris muscles. (A) Representative images of fibre type composition in soleus and plantaris muscles at different stages of growth. Type I fibres (red), type II fibres (green), and hybrid fibres (orange). Images were taken at ×100 magnification, scale bars = 200 µm. (B) Quantitative analysis of fibre type composition during development, maturation, and ageing (n = 6 mice/group). ***p < 0.0001 compared to type I fibre of plantaris muscle, ^##p < 0.001 and ^###p < 0.0001 compared to hybrid fibre/type II fibre of soleus muscle.