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. 2020 May 19;10:8239. doi: 10.1038/s41598-020-65067-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Muscle weight, body weight, and histological characteristics of soleus and plantaris muscles during development, maturation, and ageing. (A) Muscle weight, (B) Body weight, (C) Representative hematoxylin and eosin-stained images of developmental, mature, and aged muscles, (D) Quantitative analysis of fibre cross-sectional area (CSA), (E) Histogram analysis of muscle fibre size distribution, and (F) Quantitative analysis of fibre number. Images were taken at ×400 magnification, scale bars = 50 µm. ***p < 0.001 compared to developmental stage, ^#p < 0.001 compared to maturation stage (n = 6 mice/group).