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. 2020 May 19;10:8239. doi: 10.1038/s41598-020-65067-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Centronucleated muscle fibres and downstream mTOR signalling protein expression in soleus and plantaris muscles during development, maturation, and ageing. (A) Representative image of centronucleated muscle fibres, (B) Percentage of centronucleated muscle fibres at different stages of growth, (C) Phosphorylation of 4E-BP1/4E-BP1 protein expression level, and (D) Phosphorylation of p70 S6K1/p70 S6K1 protein expression level. Image was taken at ×400 magnification, scale bar = 50 µm. *p < 0.05, **p < 0.01, ***p < 0.001 compared to developmental stage and ^#p < 0.05, ^##p < 0.01 compared to maturation stage (n = 6 mice/group).