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. 2020 May 19;10:8239. doi: 10.1038/s41598-020-65067-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression and localisation of vitamin D system-related proteins in SMSC. (A) Representative image of purified SMSCs (MyoD⁺ cells), (B) Representative images of VDR and CYP24A1 protein expression in developmental, mature, and aged SMSCs. Co-localisation of VDR and CYP24A1 protein expression in the nucleus (DAPI-stained) was demonstrated in SMSCs after treated with 100 nM 1α,25(OH)₂D₃ for 24 h. (C) CYP27B1 protein expression was localised in the cytoplasmic compartment of SMSCs (arrows) in response to 100 nM 1α,25(OH)₂D₃-treated for 24 h. Images of A and B-C were taken at ×200 and ×400 magnifications, respectively.