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. 2020 May 19;10:8218. doi: 10.1038/s41598-020-65129-3

Figure 6.

Figure 6

Stage 3 NKT cells are more sensitive to CD28 blockade than Stage 2 NKT cells. (A) Number of live stage 2 and stage 3 NKT cells after co-culture with splenocytes loaded with 0.1-100 ng/mL α-GalCer. Black asterisks compare stage 2 to stage 3 while red asterisks compare media to CTLA-4Ig within stage 2 or stage 3. (B) Fold expansion of NKT cells after co-culture with splenocytes loaded with 0.1–100 ng/mL α-GalCer. (C) Mean division score of NKT cells after co-culture with splenocytes loaded with 0.1–100 ng/mL α-GalCer. All data displayed in graphs correspond to mean +/− SEM of 3 biological replicates. Statistical significance was determined using student’s t test or one-way ANOVA followed by Bonferroni tests. P values as follows: *p < 0.05, **p < 0.001, and ***p < 0.0001. Flow cytometry gating strategy outlined in Materials and Methods.