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. 2020 May 19;10:8250. doi: 10.1038/s41598-020-64931-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RCBD binds to cell surface integrins and causes decreased adherence of ARPE-19 cells to collagen as compared to CBD: (Panel A) Immunofluorescence staining of β1 integrin in ARPE-19 cells. Cells were seeded on coverslips in presence of vitreous derived from patients undergoing surgery for retinal detachment, in the absence and presence of RCBD; immunofluorescence staining was done using mouse anti-β1 integrin antibody and goat anti-mouse-FITC secondary antibody. β1 integrin-associated fluorescence is shown in green and nuclei (DAPI) in blue; scale bar: 50 µm (Panel B) Adherence of ARPE-19 cells (untreated or pre-treated with CBD and/or RCBD) on collagen coated surface; adhered cells were imaged; scale bar: 400 µm, 10X magnification; and (Panel C) Bar diagrams represent the corresponding quantitative data.