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. 2020 May 19;11:2510. doi: 10.1038/s41467-020-16321-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Silver stain analysis of purified Strep-METTL2A, 2B and 6 from human 293T cells. Arrowheads denote bands that are enriched in the METTL2A, 2B and 6 purifications relative to control. Asterisks denote non-specific contaminants found in all three purifications. b Protein matches detected through LC-MS analysis of peptides present in the indicated protein purifications. c Immunoblot analysis of control, METTL2A, METTL2B and METTL6 purifications. The immunoblot was probed with anti-TwinStrep and anti-DALRD3 antibodies. Input represents 1% of total. Asterisk represents non-specific band. Percentage DALRD3 purified represents the fraction of DALRD3 recovered from the total input in each purification. N = 1. d Coverage of the reciprocal purification of DALRD3 in protein samples analyzed by LC-MS. e Schematic of human cytosolic arginyl-tRNA synthetase (RARS), mitochondrial arginyl-tRNA synthetase (RARS2) and DALRD3. Domains are indicated representing mitochondrial targeting signal (MTS), aminoacyl synthetase catalytic core and DALR anticodon-binding domain. Source data are provided as a Source data file.