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. 2020 May 19;11:2510. doi: 10.1038/s41467-020-16321-6

Fig. 2. DALRD3 forms a complex with METTL2A/B to bind distinct arginine tRNAs.

Fig. 2

a Immunoblot of Strep-DALRD3 purified from 293T cells expressed alone or in conjunction with FLAG-METTL2A/B. Immunoblot was probed with anti-TwinStrep and anti-FLAG antibodies. Load represents a non-specific protein band detected by the anti-Strep antibody used as load control. b Nucleic acid stain of RNAs extracted from the indicated input or purified samples after denaturing PAGE. The migration pattern of 5.8S rRNA (~150 nt), 5S rRNA (~120 nt) and tRNAs (~70–80 nt) are denoted. c Northern blot analysis of the gel in b using the indicated probes. Input represents 2% of total extracts used for purification. The percentage yield represents the amount of RNA in the Strep purification that was recovered from the total input. The experiment was performed three times with comparable results. Quantification was performed on the blot shown. Source data are provided as a Source data file.