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. 2020 May 19;11:2510. doi: 10.1038/s41467-020-16321-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Immunoblot analysis of the purification of Strep-METTL2A/B expressed alone or with FLAG-DALRD3 from 293T human embryonic cells. The immunoblot was probed with anti-TwinStrep and anti-FLAG antibodies. Load represents a non-specific protein band detected by the anti-Strep antibody used as load control. Input represents 2% of total. b Nucleic acid stain of RNAs extracted from the indicated input or purified samples after denaturing PAGE. The migration pattern of tRNAs (70–80 nt), 5.8S rRNA (~150 nt), and 5 S rRNA (~120 nt) are denoted. c Northern blot analysis of METTL2A/B-associated RNAs with the indicated probes. The known presence or absence of m3C and the METTL enzyme that generates m3C in a given tRNA is denoted on the right. The purification was repeated three times with similar results. Source data are provided as a Source data file.