Figure 3.

Effect of alendronate or calcium supplement, on the bone trabecula and intertrabecular spaces in mice under long term rabeprazole treatment. Photomicrographs for bone sections stained with hematoxylin and eosin. (A) Vehicle group showing normal bone tissue with a parallel arrangement of bone trabeculae (arrow), and an arrowhead pointing to osteoclasts, (B) Rabeprazole group showing widely separated, thin-walled bone trabeculae (arrow) with widened inter trabecular spaces containing bone marrow element and apparently increase in the number of osteoclasts (Arrowhead), (C, D) Rabeprazole+Calcium group and Rabeprazole+Alendronat group showing thick bone trabeculae (arrows). Trabecular thickness was measured in random 6 points and averaged for each image. Also, arrowheads pointing to osteoclasts. (E) Column chart demonstrating the mean trabecular thickness (µm) in the experimental groups. Data are mean±SD, *Comparison to the vehicle, ^#Comparison to rabeprazole group, ^CComparison to rabeprazole+calcium group, at P-value <0.05.