Figure 5.

Effect of alendronate or calcium supplement, on the immunostaining for tartrate resistant acid phosphatase in osteoclasts in mice under long term rabeprazole treatment. Photomicrographs for bone sections immunostained for TRAP. (A) Vehicle group showing bone marrow containing osteoclast as a large cell has multiple nuclei (N), and cytoplasmic brown stained areas demonstrating the immunostaining of acid phosphatase (T), (B) Rabeprazole group showing bone marrow containing osteoclast as a large cell has multiple nuclei (N), and cytoplasmic dark brown stained areas demonstrating the immunostaining of acid phosphatase (T), (C) Rabeprazole + Calcium group showing bone marrow containing osteoclast as a large cell has multiple nuclei (N), and cytoplasmic dark brown stained areas demonstrating the immunostaining of acid phosphatase (T). (D) Rabeprazole + Alendronate group showing bone marrow containing osteoclast as a large cell has multiple nuclei (N), and cytoplasmic brown stained areas demonstrating the immunostaining of acid phosphatase (T). (E) Column chart for optical density of immunostained TRAP in the experimental groups. *Comparison to vehicle group, ^#Comparison to rabeprazole group at P-value <0.05.