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. 2020 May 13;11:583. doi: 10.3389/fphar.2020.00583

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Copyright © 2020 Shaalan, El-Sherbiny, El-Abaseri, Shoaeir, Abdel-Aziz, Mohamed, Zaitone and Mohammad

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of alendronate or calcium supplement on immunostaining for osteopontin in mice under long term rabeprazole treatment. Photomicrographs for bone marrow sections immunostained for osteopontin. (A) Vehicle group showing, bone marrow (BM), endosteum of bone (Arrows) and brown stained lines demonstrating osteopontin in bone (Arrow heads), (B) Rabeprazole group showing bone marrow (BM), endosteum of bone (Arrows) and darkly brown stained lines demonstrating osteopontin in bone (Arrow heads), (C, D) Rabeprazole + Calcium and Rabeprazole + Alendronate groups showing bone marrow (BM), endosteum of bone (Arrows) and darkly brown stained lines demonstrating osteopontin in bone (Arrow heads). (E) Column chart for area % of immunostained osteopontin. Data are mean ± SD *Comparison to vehicle group, ^#Comparison to rabeprazole group at P-value <0.05.