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. 2020 May 13;11:909. doi: 10.3389/fmicb.2020.00909

TABLE 1.

Escherichia coli strains and plasmids.

Strain or plasmid Relevant genotype or description Source or references
Strains
 MG1655 K-12, F λ ilvG rfb-50 rph-1 Blattner et al., 1997
 AM604 MC4100 ara + Wu et al., 2006
 AS19 E. coli strain B, hyperpermeable strain Sekiguchi and Iida, 1967
 NR698 imp4213 Ruiz et al., 2005
 XL1-Blue recA1 endA1 gyrA96 (NalR) thi-1 hsdR17 supE44 relA1 lac [F’ proAB lacIqZΔM15 Tn10 (TetR)] NEB
 BTH101 F cya-99 araD139 galE15 galK16 rpsL1 (StrR) hsdR2 mcrA1 mcrB1 Karimova et al., 1998; Ouellette et al., 2017
 M15/pREP4 F lac thi mtl/pREP4 QIAGEN
 BL21(DE3) F ompT gal dcm lon hsdSB(rB mB) (λDE3 [lacI lacUV5-T7 gene 1 ind1 Sam7 nin5]) Studier and Moffatt, 1986
Plasmids ori
 pSTM25 aadA Plac::t25-TM p15A Ouellette et al., 2014
 pUTM18C bla Plac::t18-TM ColE1 Ouellette et al., 2014
 pUTM18C-MalE malE sequence (residues 27–396) cloned downstream T18-TM ColE1 This study
 pSTM25-LptAm lptA sequence (residues 28–159) cloned downstream T25-TM p15A This study
 pUTM18C-LptAm lptA sequence (residues 28–159) cloned downstream T18-TM ColE1 This study
 pUTM18C-LptAmQ62L lptA sequence (residues 28–159) bearing a Q-to-L mutation at position 62 cloned downstream T18-TM ColE1 This study
 pSTM25-LptA lptA sequence (residues 28–185) cloned downstream T25-TM p15A This study
 pSTM25-LptAQ62L lptA sequence (residues 28–185) bearing a Q-to-L mutation at position 62 cloned downstream T25-TM p15A This study
 pUTM18C-LptA lptA sequence (residues 28–185) cloned downstream T18-TM ColE1 This study
 pUTM18C-LptAQ62L lptA sequence (residues 28–185) bearing a Q-to-L mutation at position 62 cloned downstream T18-TM ColE1 This study
 pST25-LptC lptC full-length sequence cloned downstream T25 p15A This study
 pUT18C-LptC lptC full-length sequence cloned downstream T18 ColE1 This study
 pQEsH-lptC pQE30 (QIAGEN) derivative, expresses His6-LptC24–191; bla Sperandeo et al., 2011
 pET-LptAΔ160–185 –H pT7-lptAΔ160–185 -His6; bla Laguri et al., 2017