TABLE 2.
Oligonucleotides.
| Name | Sequence (5'–3')a | Used to make | |
| AP576 | Reverse | attgtggatccTTAAGGCTGAGTTTGTTTG | lptC cloning in pSTM25 and pUTM18C; BamHI |
| AP579 | Forward | taatgtcgacgAAAATCGAAGAAGGTAAACTG | malE cloning in pUTM18C; SalI |
| AP580 | Reverse | aaggatctagaTTACTTGGTGATACGAGTCTGC | malE cloning in pUTM18C; XbaI |
| AP581 | Forward | gagacgagctcgGTAACCGGAGACACTGATCAG | lptA and lptAm cloning in pUTM18C; SacI |
| AP582 | Reverse | gagaggaattcTTAATTACCCTTCTTCTGTGC | lptA cloning in pUTM18C; EcoRI |
| AP665 | Reverse | gagaggaattcTTAGCGCTTGCCTTTGTCG | lptAm cloning in pUTM18C; EcoRI |
| AP666 | Forward | aaggatctagagGTAACCGGAGACACTGATCAG | lptA cloning in pSTM25; XbaI |
| AP667 | Reverse | attgtggatccTTAATTACCCTTCTTCTGTGC | lptA cloning in pSTM25; BamHI |
| AP688 | Reverse | attgtggatccTTAGCGCTTGCCTTTGTCG | lptAm cloning in pSTM25; BamHI |
| AP689 | Forward | gaagatctgcagggATGAGTAAAGCCAGACGTTG | lptC cloning in pSTM25; PstI |
| AP690 | Forward | gaagatctgcaggATGAGTAAAGCCAGACGTTG | lptC cloning in pUTM18C; PstI |
| AP733 | Forward | ATCGTCACCCTGGGCACCATC | Q62L mutagenesis in lptA |
| AP734 | Reverse | GACATTACCGGTAAAGGTAACC | Q62L mutagenesis in lptA |
aE. coli genomic sequence in uppercase; restriction sites in underlined lowercase; codon mutated by site-directed mutagenesis in bold.