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. 2020 May 13;11:836. doi: 10.3389/fimmu.2020.00836

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Copyright © 2020 Phelan, McQuaid, Kenny, Gogan, Cox, Basdeo, O’Leary, Tazoll, Ó Maoldomhnaigh, O’Sullivan, O’Neill, O’Sullivan and Keane.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DFX enhances the expression of glycolytic metabolism genes in hMDMs infected with Mtb. hMDMs, differentiated from PBMCs isolated from healthy blood donors, were stimulated with Mtb iH37Rv or infected with Mtb H37Ra for 3 h, washed to remove unphagocytosed Mtb, and were treated with DFX (100 μM). 24 h post infection transcript levels of (A,B) PFKFB3 (n = 5), (C,D) GAPDH (n = 5) (E,F), PKM2 (n = 3–5) (representing glycolysis), and (G,H) ATP5B (n = 5) (representing oxidative phosphorylation) were determined by RT-qPCR. Bars denote mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 (Two-way ANOVA with Bonferroni post hoc tests).