Figure 2.

Expression of CLEC12A on porcine leukocyte subsets. (A) Flow cytometric analysis of porcine alveolar macrophages stained with biotin-conjugated mAb FA2B10 or FA6A5 followed by streptavidin BV421. Open histograms correspond to the negative control using an irrelevant isotype-matched mAb. (B) PBMC were double stained with anti-CD3, anti-CD4, anti-CD8α, anti-CD21 or anti-CD172a mAbs, and PE-conjugated rabbit F(ab')2 anti-mouse Ig (x-axis), followed by biotin-conjugated FA2B10 and streptavidin BV421 (y-axis). Isotype-matched irrelevant mAbs, unlabeled or biotin-labeled were used as negative controls. For analysis, cells were gated as FSC^hi (region R) to enrich the cDC population. Numbers indicate the percentage of cells within the respective quadrants. Results are representative of three independent experiments.