Figure 3.

Expression of CLEC12A on plasmacytoid dendritic cells (pDCs). PBMC were first stained with anti-CD172a mAb BA1C11 and anti-CD4 mAb 74-12-4 followed by APC-conjugated goat anti-mouse IgG1 and PE-conjugated goat anti-mouse IgG2b. After blocking free binding sites, cells were incubated with biotin-labeled anti-CLEC12A mAb FA2B10 and streptavidin BV421. After doublet exclusion, cells with high FSC and SSC were selected and pDCs were identified as the CD172a^lo CD4^hi subpopulation. CLEC12A expression in gated population is shown in the filled histogram; open histogram corresponds to the negative control with an irrelevant isotype-matched mAb. The profiles shown are from a representative experiment out of three performed with cells from different donors.