Figure 4.

CLEC12A expression on blood conventional dendritic cells (cDCs). PBMC were first incubated with anti-CADM1 (3E1), anti-CD172a (74-22-15) and biotin-labeled anti-CLEC12A (FA2B10) mAbs followed by PE-conjugated goat anti-mouse IgG2b and Alexa Fluor 647-conjugated goat anti-chicken IgY. After blocking free binding sites, cells were incubated with FITC-conjugated anti-CD14 mAb MIL-2 and streptavidin BV421. Cells with high FSC and SSC were selected. Among these cells, cDC1 were gated as CD14⁻ CD172a^lo CADM1⁺, cDC2 as CD14⁻ CD172a^hi CADM1⁺, and monocytes as CD14⁺. Expression of CLEC12A in gated populations is shown as filled histograms; open histograms correspond to negative controls using an irrelevant isotype-matched mAb. The profiles shown are from a representative experiment out of three performed with cells from different donors.